The Department of Neurosurgery at Stanford University is seeking an independent and motivated full-time Life Science Research Professional 1 working in the field of spatial transcriptomics. S/he will perform basic functions and activities involved in defined research projects, independently conduct and analyze experiments, and assist with lab management.
The LSRP1 will work under general guidance from the Principal Investigator and lab members to assist in research on the cell-cell communication in the rodent nervous system and/or in brain tumor. The position involves diverse experimental approaches including isolation/culture of primary human cells, molecular biology (e.g. single-molecule FISH, immunostaining, cloning, qPCR), animal surgery, and imaging. Additional day-to-day lab functions include ordering supplies, maintaining lab stocks, and assisting the Principal Investigator with regulatory requirements and lab databases. On the job training in specific techniques will be provided, but prior experience with related techniques is desirable but not required. Applicant should be organized, detail-oriented, intellectually motivated and have excellent communication skills.
The goal of this laboratory is to develop optical imaging-based approaches to understand the genetic basis underlying the complex cellular phenotypes and communication in the central nervous system, and how genetic anomality contributes to CNS disease and cancer.
Duties include:
- Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
- Independently conduct experiments; maintain detailed records of experiments and outcomes.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
- Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
- Assist with orientation and training of new staff or students on lab procedures or techniques.
DESIRED QUALIFICATIONS:
- Prior experience with rodent perfusions, tissue dissections, harvesting, cryosectioning, and histology/imaging is preferable.
- Prior experience with single cell analysis, spatial transcriptomics analysis, python, R is highly desired.
- Prior experience with a range of experimental techniques, including cell culturing, molecular biology methods (e.g., Western blotting, immunostaining, qPCR, in vitro transcription, reverse transcription, DNA/RNA purification) is highly desired.
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
PHYSICAL REQUIREMENTS:
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
WORKING CONDITIONS:
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
WORK STANDARDS:
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University’s Administrative Guide.
