Stanford University is seeking a Research Assistant (Life Science Research Professional 1) to perform basic functions and activities involved in defined research projects, and independently conduct and analyze experiments.
We are seeking a full time Research Assistant (Life Science Research Professional 1) to join the Buckwalter Laboratory in the Department of Neurology at Stanford University. The LSRP1 will work in a small, welcoming, and supportive group studying axon-glial and axon-immune interactions after stroke using mouse models. The LSRP1 will aid in the investigation of differences in the neuroimmune response to stroke between animals resistant to axonal degeneration after stroke, and how this affects long term cognitive outcomes. Experience with mouse handling/animal behavior and/or immunohistochemistry is highly desirable. Experience with flow cytometry, axonal/myelin biology or lymphocyte biology, and RNA sequencing are desirable. The job would entail performance of independent research as well as 5-10% effort on lab management tasks that are shared across all lab members. Excellent communication and collaborative skills and a 1.5 year commitment are essential.
Duties include*:
- Perform complex experiments for a major portion of the research project. Maintain detailed records of experiments and outcomes.
- Interpret and analyze results, often where few guidelines exist. Based on prior results, contribute to development of new research protocols and procedures or adaptation of existing protocols.
- Make recommendations on plan design and/or research direction to principal investigator.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute substantively to the preparation of papers for publication, especially in the results section. May contribute to publication of findings and present ongoing work and findings to colleagues at academic conferences.
- Supervise (either formally or informally) staff or students as needed, including oversight and instruction on techniques, as well as consultation on project work. Serve as technical resource for other research staff.
- Perform general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed. May assist with purchasing laboratory equipment.
DESIRED QUALIFICATIONS:
The desired applicant will have a bachelor’s degree in biochemistry, cell biology, biological sciences, neuroscience, or related field. The candidate should be able to work independently, be highly motivated, well organized, adaptable and work well within a highly interdisciplinary and collaborative team. Good communication and interpersonal skills as well as fluent English are essential, as well as proficient computer skills (Mac and PC).
Prior experience in a lab environment is important. Specific techniques used in the lab that would be valuable include:
- Mouse behavior assays such as Barnes maze and novel object tasks.
- Immunohistochemistry and Immunofluorescence staining procedures.
- Confocal microscopy.
- RNA preparation from tissues, qPCR, RNASeq library preparation and analysis.
- Experience working with blood from mammalian sources.
- Running a flow cytometer and analyzing flow cytometry data (FlowJo and cytobank.org) as well as other forms of (antibody-based) immuno-assays (ELISA, IP, bead-based assays).
- Solid working knowledge of statistics.
- A minimum 1.5 year commitment is highly desired.
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
PHYSICAL REQUIREMENTS*:
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
WORKING CONDITIONS:
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
- May require working directly with live animals including mice and rats.
WORK STANDARDS:
- Interpersonal Skills: Demonstrates the ability to work well with Stanford colleagues and clients and with external organizations.
- Promote Culture of Safety: Demonstrates commitment to personal responsibility and value for safety; communicates safety concerns; uses and promotes safe behaviors bases on training and lessons learned.
- Subject to and expected to comply with all applicable University policies and procedures, including but not limited to the personnel policies and other policies found in the University’s Administrative Guide.
