The Bassik Lab at Stanford University (http://bassiklab.stanford.edu) is seeking a Life Science Research Professional 1 to be an integral member of a research team that is developing novel genetic tools, including ultracomplex CRISPR/Cas9 libraries, to conduct genetic screens in a range of medically relevant areas, such as cancer, neurodegeneration and infectious disease. The goal of this work is to understand (1) the biology of cancer development and identify novel therapeutic drug targets, as well as to (2) understand how macrophage cells of the immune system recognize and destroy pathogens ranging from protein aggregates to tumor cells. Candidate must be willing to work in a research team, be organized, enjoy problem solving, and have strong communication skills. This is a fantastic opportunity for a motivated, creative, and reliable person to participate in highly collaborative, cutting-edge research at the forefront of modern biology, with real world applications to develop new therapeutics to improve human health.
Responsibilities include cell culture and maintenance of human cell lines, basic molecular biology (PCR, cloning, running DNA and protein gels), flow cytometry, preparation of samples for deep sequencing, confocal microscopy, basic statistical calculations, and other duties as assigned. The LSRP 1 will be expected to work closely with the PI to develop protocols, interpret and analyze the results of experiments, and suggest modifications to procedures as appropriate. The position includes lab management tasks such as ordering supplies, maintaining lab records, and adhering to safety regulations. The LSRP 1 is expected to keep abreast of scientific literature and contribute new ideas and techniques to the lab, as well as attend lab meetings and journal clubs.
Duties include:
- Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
- Independently conduct experiments; maintain detailed records of experiments and outcomes.
- Primary experiments involve cell culture, molecular biology, high-throughput CRISPR screening, high-throughput sequencing and analysis.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
- Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
- Assist with orientation and training of new staff or students on lab procedures or techniques.
DESIRED QUALIFICATIONS:
- Proficiency with basic molecular biology techniques, such as gel electrophoresis, PCR, and recombinant DNA techniques.
- Experience with mammalian tissue culture techniques.
- Meticulous record keeping and organizational skills.
- Experience with high throughput sequencing methods and sample preparation, fluorescence microscopy, flow cytometry, lentivirus handling, and computer programming.
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge of science principles to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
CERTIFICATIONS & LICENSES:
None.
PHYSICAL REQUIREMENTS:
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
WORKING CONDITIONS:
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
Stanford is an equal employment opportunity and affirmative action employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, protected veteran status, or any other characteristic protected by law.
