The laboratory of Dr. Andreas Tolias is seeking a talented LSRP1 to join a multi-disciplinary research team aimed at understanding the cellular diversity of brain circuits. The specific goals of these positions will be to use whole-cell patching methods, single-cell RNA-sequencing and immunochemistry to probe morphology, electrophysiology and transcriptome of the individual neurons of brain circuits across cortical areas and species including humans. In particular, Patch-seq, a method we recently developed, that combines whole-cell patch-clamp recordings and single-cell RNA sequencing (scRNA-seq). The individual will receive extensive one-on-one training with postdocs and junior faculty on cellular electrophysiology to develop the necessary skills to acquire high-quality data. They will also have opportunity of learn in vivo multi-photon imaging and multi-electrode recordings using Neuropixels and advance their computational and programming skills.
The ideal applicants should have some experience in cellular electrophysiology, PCR or RNA-sequencing. The candidates without these experiences will be also considered if they are willing to commit the efforts to develop these skills.
Duties Include:
- Plan approach to experiments in support of research projects in lab and/or field based on knowledge of scientific theory.
- Independently conduct experiments; maintain detailed records of experiments and outcomes.
- Apply the theories and methods of a life science discipline to interpret and perform analyses of experiment results; offer suggestions regarding modifications to procedures and protocols in collaboration with senior researcher.
- Review literature on an ongoing basis to remain current with new procedures and apply learnings to related research.
- Contribute to publication of findings as needed. Participate in the preparation of written documents, including procedures, presentations, and proposals.
- Help with general lab maintenance as needed; maintain lab stock, manage chemical inventory and safety records, and provide general lab support as needed.
- Assist with orientation and training of new staff or students on lab procedures or techniques.
*- Other duties may also be assigned
DESIRED QUALIFICATIONS:
Master’s degree in a basic science. One year of directly related experience with patch-clamp.
EDUCATION & EXPERIENCE (REQUIRED):
Bachelor's degree in related scientific field.
KNOWLEDGE, SKILLS AND ABILITIES (REQUIRED):
- General understanding of scientific principles. Demonstrated performance to use knowledge and skills when needed.
- Demonstrated ability to apply theoretical knowledge of science principals to problem solve work.
- Ability to maintain detailed records of experiments and outcomes.
- General computer skills and ability to quickly learn and master computer programs, databases, and scientific applications.
- Ability to work under deadlines with general guidance.
- Excellent organizational skills and demonstrated ability to accurately complete detailed work.
CERTIFICATIONS & LICENSES:
None
PHYSICAL REQUIREMENTS*:
- Frequently stand, walk, twist, bend, stoop, squat, grasp lightly, use fine manipulation, grasp forcefully, perform desk-based computer tasks, use telephone, write by hand, lift, carry, push and pull objects weighing over 40 pounds.
- Occasionally sit, kneel, crawl, reach and work above shoulders, sort and file paperwork or parts.
- Rarely climb, scrub, sweep, mop, chop and mix or operate hand and foot controls.
- Must have correctible vision to perform duties of the job.
- Ability to bend, squat, kneel, stand, reach above shoulder level, and move on hard surfaces for up to eight hours.
- Ability to lift heavy objects weighing up to 50 pounds.
- Ability to work in a dusty, dirty, and odorous environment.
- Position may require repetitive motion.
WORKING CONDITIONS:
- May require working in close proximity to blood borne pathogens.
- May require work in an environment where animals are used for teaching and research.
- Position may at times require the employee to work with or be in areas where hazardous materials and/or infectious diseases are present.
- Employee must perform tasks that require the use of personal protective equipment, such as safety glasses and shoes, protective clothing and gloves, and possibly a respirator.
- May require extended or unusual work hours based on research requirements and business needs.
